U.S. AI Companies That Raised $100M+ in 2025 So Far

In 2024, 49 startups secured funding rounds of $100 million or more, according to TechCrunch data. Among them, three companies managed to raise multiple “mega-rounds,” while seven reached or exceeded the $1 billion milestone.

As 2025 advances, the momentum appears to be carrying forward. With only a few weeks left in the third quarter, the pace of billion-dollar rounds remains strong, and several AI startups have already raised more than one mega-round this year.

Below is a detailed breakdown of all the U.S.-based AI startups that raised $100 million or more in 2025:

August

• EliseAI: The healthcare and housing automation platform raised $250 million (Series E) on August 20, valuing the startup at $2.2 billion. Andreessen Horowitz led the round.
• Decart: An AI research lab, secured $100 million at a $3.1 billion valuation on August 7. The round included Sequoia Capital, Benchmark, and Zeev Ventures.

July

• Fal: The generative media platform raised $125 million (Series C) on July 31 at a $1.5 billion valuation. Meritech Capital Partners led the round, joined by Salesforce Ventures, Shopify Ventures, and Google AI Futures Fund.
• Ambience Healthcare: Building an AI-powered healthcare operating system, the startup raised $243 million (Series C) led by Oak HC/FT and Andreessen Horowitz, with Kleiner Perkins and OpenAI Startup Fund participating.
• Reka AI: Raised $110 million (Series B) on July 22, reaching a $1 billion valuation. Investors included Snowflake and Nvidia.
• Thinking Machines Lab: Confirmed a $2 billion seed round on July 15, led by Andreessen Horowitz with Nvidia, Accel, and AMD joining. Valuation: $12 billion.
• OpenEvidence: Based in Cambridge, Massachusetts, the AI-powered clinician search tool raised $210 million (Series B) at a $3.5 billion valuation on July 15. Kleiner Perkins and GV led the round.
• Harmonic: Focused on a mathematical reasoning engine, it raised $100 million (Series B) on July 10, led by Kleiner Perkins. Valuation: $875 million.

June

• Abridge: Healthcare AI unicorn raised $300 million (Series E), valuing it at $5.3 billion. Andreessen Horowitz and Khosla Ventures participated. This was Abridge’s second raise of 2025.
• Harvey: Legal AI startup raised its second $300 million round of 2025 (Series E) on June 23, co-led by Kleiner Perkins and Coatue. Valuation: $5 billion.
• Tennr: Healthcare AI platform raised $101 million (Series C) led by IVP, with Lightspeed and GV joining. Valuation: $605 million.
• Glean: Enterprise AI search startup secured $150 million (Series F) on June 10, led by Wellington Management. Valuation: $7.25 billion.
• Anysphere: Developer of AI coding tool Cursor raised $900 million (Series C) on June 10, led by Thrive Capital with Andreessen Horowitz and Accel participating. Valuation: nearly $10 billion.

May

• Snorkel AI: Data labeling startup raised $100 million (Series D) on May 29 at a $1.3 billion valuation. Addition led the round.
• LMArena: AI model benchmarking platform raised a $100 million seed round on May 21 at a $600 million valuation. Andreessen Horowitz and UC Investments co-led the raise.
• TensorWave: Las Vegas-based AI infrastructure company raised $100 million (Series A) on May 14. Magnetar Capital and AMD Ventures co-led.

April

• SandboxAQ: Closed a $450 million (Series E) round on April 4, valuing the company at $5.7 billion. Investors included Nvidia, Google, and Ray Dalio.
• Runway: AI media production platform raised $308 million (Series D) on April 3 at a $3 billion valuation, led by General Atlantic with Nvidia and SoftBank joining.

March

• OpenAI: Closed a record-breaking $40 billion funding round on March 31, valuing the company at $300 billion. The round was led by SoftBank, with Microsoft and Coatue among others.
• Nexthop AI: Infrastructure company raised $110 million (Series A) on March 25, led by Lightspeed Venture Partners.
• Insilico Medicine: Based in Cambridge, the company raised $110 million (Series E) on March 13 for its generative AI drug discovery platform. Valuation: $1 billion.
• Celestial AI: Infrastructure company raised $250 million (Series C) on March 11, valuing it at $2.5 billion. Fidelity led the round.
• Lila Sciences: Raised $200 million (seed) to develop a science superintelligence platform. Investors included Flagship Pioneering and General Catalyst.
• Reflection.Ai: Brooklyn-based startup raised $130 million (Series A), valuing it at $580 million. Lightspeed and CRV led.
• Turing: AI coding startup raised $111 million (Series E) on March 7, valuing it at $2.2 billion.
• Shield AI: Defense AI startup raised $240 million (Series F) on March 6, co-led by L3Harris and Hanwha Aerospace. Valuation: $5.3 billion.
• Anthropic: Raised $3.5 billion (Series E) on March 3, valuing the company at $61.5 billion. Lightspeed led with Salesforce Ventures joining.

February

• Together AI: Open-source AI development platform raised $305 million (Series B) on February 20 at a $3.3 billion valuation.
• Lambda: AI infrastructure startup raised $480 million (Series D), valuing it at $2.5 billion. Nvidia and ARK Invest participated.
• Abridge: Secured $250 million (Series D) on February 17 at a $2.75 billion valuation.
• Eudia: Legal AI company raised $105 million (Series A) on February 13.
• EnCharge AI: AI hardware startup raised $100 million (Series B) on February 13, led by Tiger Global and Samsung Ventures.
• Harvey: Raised $300 million (Series D) on February 12, valuing it at $3 billion.

January

• ElevenLabs: Synthetic voice startup raised $180 million (Series C) on January 30, valuing the company at $3 billion+. ICONIQ Growth and Andreessen Horowitz co-led.
• Hippocratic AI: Healthcare-focused LLM company raised $141 million (Series B) on January 9, valuing it at $1.6 billion.